Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 14 pg/mL,
Range:6.86 - 5000 pg/mL,
Assay Platform:Microplate
Product details:
Abcam's Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA
range.
NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.
Biological function summary
Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.
Pathways
Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.
Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.
