Agilent, 122-5732, J&W DB-5HT GC column, 30 m, 0.25 mm, 0.25 µm, 7 inch cage

The Agilent J&W DB-5HT capillary GC column combines a low-polarity stationary phase with high-temperature fused-silica tubing for gas chromatographic separations. Its dimensions are 30 m in length, 0.25 mm internal diameter, and 0.25 µm film thickness, supplied on a 7-inch cage. The low-polarity phase is suited to analytes that separate predominantly through volatility and dispersive interactions, including many nonpolar to moderately polar mixtures. The column is designed for a specified temperature range extending up to 400 °C, making thermal limits an important method-development consideration. The 0.25 mm bore and 0.25 µm film balance separation efficiency, sample capacity, and retention under appropriate carrier-gas conditions. The 7-inch cage fits common GC oven configurations. Column conditioning, inlet cleanliness, oxygen control, and the chosen temperature program directly influence lifetime and reproducibility.