Agilent, 227415-702, Altura Oligo HPH-C18 column 2.7μm, 4.6x150mm
Altura Oligo HPH-C18 2.7um, 4.6x150mm
Catalog Number: 227415-702
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| Brand | Altura Oligo HPH-C18 |
|---|---|
| Guard Column | No |
| Hardware | Ultra Inert SS |
| Inner Diameter (ID) | 4.6 mm |
| LC Platform | BioInert |
| Length | 150 mm |
| Maximum Temperature | 65 °C |
| Particle Size | 2.7 µm |
| Particle Type | Superficially Porous |
| Phase | HPH-C18 |
| Pore Size | 100 Å |
| Pressure Rating | 600 bar |
| Separation Mode | Reversed Phase |
| Shipping Solvent | Acetonitrile/Water |
| Storage Temp Conditions | Store at ambient temperature |
| Technique | LC & LC/MS |
| Weight | 105 g |
| pH Range | 3-11 |
Answer:The Altura Oligo HPH-C18 column is 4.6 mm ID × 150 mm long and contains 2.7 µm superficially porous particles.
Answer:It uses high-pH-stable C18 reversed-phase chemistry with Ultra Inert stainless-steel hardware optimized for oligonucleotide analysis.
Answer:The listed pH range is 3–11, with a maximum temperature of 65 °C and maximum pressure of 600 bar; observe the lower limit imposed by the full system.
The Altura Oligo HPH-C18 column is a reversed-phase liquid chromatography column developed for analytical separation of oligonucleotides. It combines 2.7 µm superficially porous particles with a high-pH-stable C18 chemistry and low-adsorption Ultra Inert stainless-steel hardware. The 4.6 mm internal diameter and 150 mm length provide an analytical format for evaluating oligonucleotide purity, related species and chromatographic method selectivity. Its published operating envelope includes a 100 Å pore size, pH 3–11, maximum temperature of 65 °C and maximum pressure of 600 bar, subject to the complete method and instrument limits. Condition the column with compatible mobile phases, use suitable ion-pair or other validated oligonucleotide separation conditions and avoid abrupt solvent changes that could disrupt performance. Monitor pressure, peak shape, recovery and carryover with representative standards during method development and routine system suitability checks.
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