Agilent, 600111, T3 RNA Polymerase

Catalog Number: 600111

Pricing

Concentration	50 U/µL
Incubation Time	Incubate 30 minutes at 37 °C
Reaction Conditions	40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 10 units enzyme in a 25 µL volume. Incubate 30 minutes at 37°C
Reactions per Kit	5000 units
Storage Buffer	20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50 % glycerol (v/v)
Unit Definition	One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C

Ordering

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.