Agilent, 651750-902, AdvanceBio Peptide Mapping 120Å, 2.1 x 250 mm, 2.7 µm
AdvanceBio Peptide Map, 2.1x250mm, 2.7um
Catalog Number: 651750-902
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| Brand | AdvanceBio Peptide Mapping |
|---|---|
| Hardware | SS |
| Inner Diameter (ID) | 2.1 mm |
| LC Platform | Stainless Steel |
| Length | 250 mm |
| Maximum Temperature | 60 °C |
| Part Number Application | Peptide Mapping |
| Part Number Application Domain | Biopharma |
| Particle Size | 2.7 µm |
| Particle Type | Superficially Porous |
| Phase | C18 |
| Pore Size | 120 Å |
| Pressure Rating | 600 bar |
| Separation Mode | Reversed Phase |
| Shipping Solvent | Acetonitrile/Water |
| Technique|MultiLine | LC|LC & LC/MS| |
| UNSPSC Code | 41115711 |
| pH Range | 2-8 |
Answer:It uses reversed-phase C18 chemistry on superficially porous particles.
Answer:The column is 2.1 × 250 mm with 2.7 µm particles and 120 Å pores.
Answer:It separates enzymatically generated peptides in peptide mapping and biopharmaceutical characterization workflows.
The Agilent AdvanceBio Peptide Mapping analytical column is a reversed-phase C18 column designed for separation of peptides produced by enzymatic digestion of therapeutic proteins and other complex samples. Its 2.1 mm internal diameter and 250 mm length provide an extended separation path, while 2.7 µm superficially porous particles with 120 Å pores support high-efficiency peptide resolution. The stainless-steel hardware is suited to compatible LC and LC/MS workflows for sequence coverage studies, peptide profiling and evaluation of protein modifications. Method development should consider gradient slope, mobile-phase additives, sample load and temperature because these variables strongly affect peptide retention and peak shape. The operating envelope includes a pH range of 2–8 and a maximum temperature of 60 °C; apply the pressure limits of the column and instrument together. A matched peptide-mapping guard column helps protect the analytical bed from strongly retained contaminants.
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