Agilent, GK80090, AdvanceBio O-Glycanase

Name: AdvanceBio O-Glycanase
Brand: Agilent
SKU: GK80090

AdvanceBio O-Glycanase

Catalog Number: GK80090

Pricing

Application	Detection of O-glycosylation in proteins
Concentration	≥1.25 U/mL
Enzyme Applications\|MultiLine	Analysis of biosynthesis of glycoproteins\|Bioactivity in O-glycans\|Detection of O-glycosylation in proteins\|Epitope and binding site analysis\|Glycoprotein deglycosylation\|Structural analysis of O-glycan\|
Enzyme Formulation	20 mM Tris HCl pH 7.5 containing 25 mM NaCl.
Enzyme Source	Recombinant gene from Streptococcus pneumoniae, expressed in E.coli.
Enzyme Specific Activity	≥12 units/mg
Enzyme Specificity	The enzyme is highly specific, liberating Galß1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. O-Glycanase is included in the Agilent Enzymatic Deglycosylation kit for N-linked and simple O-linked glycans (GK80110), which may be used in conjunction with our the Agilent Extender kit for complex O-linked glycans (GK80115).

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