Agilent, GKX-5013, β(1-3,4)-Galactosidase (bovine testis)
B(1-3,4)-Galactosidase (bovine testis)
Catalog Number: GKX-5013
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| Concentration | 5 U/mL |
|---|---|
| Enzyme Applications | The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of ß(1-3) and ß(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal ß(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards ß(1-3) and ß(1-4)-linked galactose contrasts with that of our other ß-galactosidases (e.g. from S. pneumoniae, GKX-5014, or Jack bean, GKX-5012) which exhibit a preference for Gal ß(1-4), and cleave the Gal ß(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing ß galactose residues. |
| Enzyme Formulation | 20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0) |
| Enzyme Source | Bovine testis. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP-ß-D-galactopyranoside per minute at pH 4.0 and 37°C. |
| Molecular Weight | ~68 kD |
| Unit | 0.5 U |
| Volume | 100 µL |
| pH Optimum | 4 |
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