BD, 550342, Purified Rat IgM, κ Isotype Control R4-22

The R4-22 monoclonal antibody has a Rat IgM, κ isotype and has no known specificity.
This antibody has been tested by ELISA (~2 μg/ml), and Flow cytometry to assure specificity and reactivity. For immunohistochemical staining of acetone-fixed frozen sections or paraffin embeded , a dilution range of 1:12.5 - 1:50 is suggested.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.