Answer:They are used to validate the FL4 fluorescence detector on the BD Accuri C6.
Answer:The 6-peak beads assess FL4, while the 8-peak beads are used for FL1, FL2, and FL3.
Answer:The beads are approximately 3.0–3.4 µm in diameter and are in a 2 × 4 mL package.
BD Accuri Spherotech 6-Peak Validation Beads are fluorescent particle controls for assessing the FL4 detection channel of the BD Accuri C6 flow cytometer. The bead mixture creates a multi-peak fluorescence distribution that helps evaluate detector sensitivity, resolution, and response linearity during routine instrument performance checks. FL4 is the channel commonly associated with APC detection on the Accuri C6. The six-peak reagent is distinct from the eight-peak bead control used for FL1, FL2, and FL3. The bead particle size is approximately 3.0–3.4 µm, and the package contains two 4 mL containers. Performance validation uses the corresponding BD Accuri C6 acquisition and analysis templates, allowing laboratories to compare the expected fluorescent peak pattern across instrument checks. These beads are instrument setup and performance controls rather than biological cell-staining reagents or fluorescence compensation beads. They are for research use and should be evaluated using the relevant instrument software guide and validation procedure.
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