| Brand | BD™ |
|---|---|
| Alternative Name | MME; CALLA; EPN; NEP; neprilysin; SFE; atriopeptidase; enkephalinase |
| Reactivity | Human |
| Isotype | Mouse BALB/c IgG1, κ |
| Immunogen | Acute CALLA Leukemia Blast Cells |
| Application | Flow cytometry |
| Concentration | 50 µg/mL |
| Vol. Per Test | 5 μL |
| Workshop Number | V CD10.7 |
| Entrez Gene ID | 4311 |
| RRID | AB_2870372 |
| Regulatory Status | RUO (GMP) |
Answer:It binds human CD10, a cell-surface metalloprotease also known as CALLA or neutral endopeptidase.
Answer:The APC-H7 conjugate provides 100 tests at 5 µL per test.
Answer:Protect the conjugate from light, store at 2–8°C without freezing, and minimize prolonged paraformaldehyde exposure.
BD APC-H7 Mouse Anti-Human CD10 is a directly conjugated monoclonal antibody for detecting CD10 expression on human cells by flow cytometry. Clone HI10a recognizes CD10, a cell-surface metalloprotease also called common acute lymphoblastic leukemia antigen (CALLA) or neutral endopeptidase. CD10 expression helps characterize B-cell maturation, germinal-center populations, hematopoietic precursors, and selected leukocyte subsets in research panels. The APC-H7 tandem fluorochrome is excited by a red laser, allowing CD10 measurement alongside markers occupying other spectral channels. Use appropriate single-stain compensation controls and titrate the antibody within the complete multicolor panel. This formulation provides 100 tests at 5 µL per test. Store at 2–8°C, protect from light, and do not freeze. Prolonged exposure to paraformaldehyde or bright light can alter APC-H7 emission; minimize fixation time and verify compensation after any processing change.
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