Description
The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin. It also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. 9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion mediated by activated, but not unactivated, α4β1-integrin. The source of the immunogen was mouse lymph node-derived endothelial cell line TME.
Immunohistochemistry: The 9EG7 antibody specific for mouse CD29 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and Zinc-fixed paraffin sections. Tissues tested were mouse spleen and thymus. The antibody stains lymphocytes and endothelial cells. The isotype control recommended for use with this antibody is purified rat IgG2a (559073). For optimal indirect immunohistochemical staining, the 9EG7 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone 9EG7 is not recommended for formalin-fixed paraffin embedded sections.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. An isotype control should be used at the same concentration as the antibody of interest.
5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
