Description
TMB Preparation: Prepare a working concentration of TMB substrate solution within 15 minutes prior to use by mixing equal volumes of Substrate Reagent A and Substrate Reagent B (e.g. for one 96-well plate, a 12 mL TMB substrate working solution can be prepared by mixing 6 mL of Substrate Reagent A with 6 mL of Substrate Reagent B). After mixing, the solution should be colorless to a very faint blue. In the presence of peroxidase-labeled conjugates, the solution should develop a definitive blue color. After the addition of Stop Solution, the color changes from blue to yellow. Warning: Assay Diluent (component 51-2641KC) contains 0.003% (w/w) and Wash Concentrate (20X) (component 51-9003739) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1). Hazard statements May cause an allergic skin reaction. Precautionary statements Wear protective gloves / eye protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Dispose of contents/container in accordance with local/regional/national/international regulations. Danger: Substrate Reagent B (component 51-2607KZ) contains 33.05% methanol (w/w). Hazard statements Flammable liquid and vapour. Toxic if swallowed, in contact with skin or if inhaled. Causes damage to the central nervous system. Route of exposure: Oral. Precautionary statements Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Wear protective gloves / eye protection. Wear protective clothing. Wash contaminated clothing before reuse. Do not breathe mist/vapours/spray. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. IF exposed or concerned: Call a POISON CENTER/doctor. In case of fire: Use suitable extinguishing media to extinguish Danger: Stop Solution (component 51-2608KZ) contains 15.23% phosphoric acid (w/w). Hazard statements Causes severe skin burns and eye damage. Precautionary statements Wear protective gloves / eye protection. Wear protective clothing. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing. Dispose of contents/container in accordance with local/regional/national/international regulations.
Before use, bring all reagents to room temperature (18-25°C). Immediately after use, return to proper storage conditions.
1. ProClin is a trademark of Rohm and Haas Company.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
