BD, 550840, Purified Mouse Anti-HPV-16 L1 CAMVIR-1

More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33.
Immunohistochemistry: For optimal indirect immunohistochemical staining, this antibody should be titrated, such as a 1:10 to 1:50 dilution, and visualized via a three-step staining procedure in combination with polyclonal Biotin Goat Anti-Mouse Ig (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, investigators may be interested in the BD Pharmingen™ Anti-Mouse Ig HRP Detection Kit (Cat. No. 551011).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.