BD, 551091, Purified Mouse Anti-MLH-1 with Control

Applications include western blot analysis (0.5-2.0 µg/ml) and immunohistochemical staining of frozen and paraffin-embedded tissue sections (5-20 µg/ml). Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot control (Comp. No. 51-16526N; store lysate at -20°C). Additional Jurkat control lysate (Cat. No. 611451) is sold separately. Intestine or normal colon is suggested as a positive control for immunohistochemical staining. In intestine, staining is primarily nuclear and is seen in the crypts of Lieberkuhn, similar to that described in the literature. Both nuclear and cytoplasmic staining have been observed in a variety of other normal and tumor tissue and cell types. This clone is also available as Cat. No. 550838 in our special formulation for IHC application. Clone G168-728 (Cat. No. 554073) is recommended for immunoprecipitation of MLH1.
Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.