Description
ELISA Standard: The recombinant human IP-10 (Cat. No. 551130) is useful as a quantitative standard for a sandwich ELISA for measuring human IP-10 protein levels. Purified 4D5 antibody (Cat. No. 555046) can be paired with the biotinylated 6D4 antibody (Cat. No. 555048) with recombinant human IP-10 as the standard. To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~1,000 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring human-IP-10 in serum or plasma our BD OptEIA™ Human IP-10 Set (Cat. No. 550926) is specially formulated and recommended. Note: Biological Activity: This preparation of recombinant human IP-10 protein has not been evaluated for biological activity. **Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
