BD, 551506, Biotin Anti-Mouse IFN-γ R4-6A2

Name: Biotin Anti-Mouse IFN-γ R4-6A2
Brand: BD
SKU: 551506

0.5 mg

Catalog Number: 551506

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Substrate Solutions

TagsReagents immunoassay-reagents

CollectionsBD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels BD Cell Preparation & Separation Reagents (PBMC, Magnetic, RBC Lysis)

Description

The R4-6A2 antibody reacts with mouse interferon-γ (IFN-γ). The immunogen used to generate the R4-6A2 hybridoma was partially purified mouse IFN-γ protein. This is a neutralizing antibody.
ELISA Detection: The R4-6A2 antibody has been found to be useful in sandwich ELISAs that measure mouse IFN-γ protein levels. The Biotin Anti-Mouse IFN-γ antibody (Cat. No. 551506) can be used as a detection antibody along with Purified Rat Anti-Mouse IFN-γ (Cat. No. 551309) as the capture antibody and Recombinant Mouse IFN-γ (Cat. No. 554587) as the standard. The Biotin Anti-Mouse IFN-γ antibody should be titrated 0.5 -2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of mouse IFN-γ ranging from ~500 to 5 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT." For maximal sensitivity, an overnight incubation (4°C) of samples/standards with the coated capture antibody is suggested. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IFN-γ in serum or plasma our Mouse IFN-γ (AN-18) ELISA Set (Cat. No. 551866) or Mouse IFN-γ ELISA Kit II (Cat. No. 558258) are specially formulated and recommended.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Specifications

General

Source	Mouse Siglec E, amino acids (Gln20-Phe355) (Accession# NP_112458.2), was expressed in 293E with a C-terminal human IgG1-Fc tag.
Molecular Mass	This 570 amino acid recombinant protein has a predicted molecular mass of approximately 63 kD. The protein migrates at about 80 - 90 kD by SDS-PAGE in DTT-reducing conditions and about 180 - 200 kD in non-reducing conditions. The predicted N-terminal amino acid is Gly.
Purity	>95%, as determined by Coomassie stained SDS-PAGE.
Formulation	0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level	Less than 1.0 EU per µg of protein as determine by the LAL method.
Concentration	25 µg size is bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

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BD, 551506, Biotin Anti-Mouse IFN-γ R4-6A2

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