Description
The MABTNFR1-A1 antibody reacts with the extracellular domain of the 55 kDa receptor for the human cytokines: TNF (aka tumor necrosis factor-alpha , TNF-α) and lymphotoxin-alpha (LT-α3, aka, lymphotoxin or TNF-β). This receptor is referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI) or CD120a. TNFRI are expressed by a variety of cell lines, tumor cells, and normal cell types including T cells, monocytes, macrophages, neutrophils, endothelial cells, hepatocytes, chondrocytes, and fibroblasts. Naive B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes and platelets are uniformly negative for TNFRI expression. For the generation of the MABTNFRI-B1 hybridoma, BALB/c mice were immunized with purified, full-length human TNFRI protein. MABTNFRI-A1 specifically binds to natural and recombinant truncated forms of TNFRI.
ELISA Capture: The purified MABTNFR1-A1 antibody (Cat. No. 552535) is useful as a capture antibody for a sandwich ELISA which measures TNFRI protein levels. Purified MABTNFR1-A1 antibody can be paired with the biotinylated MABTNFR1-B1 anti-human TNFRI (Cat. No. 550900) as the detecting antibody, with recombinant soluble human TNFRI as the standard. Purified MABTNFR1-A1 antibody should be titrated between 1 - 4 µg/ml to determine the optimal coating concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant human TNFRI ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, visit the online protocols, or the chapter on ELISA in the Immune Function Handbook, both of which are on our web site at www.bdbiosciences.com.. Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, Lt-α, TNFRII, GM-CSF, TGFβ, This ELISA antibody pair also shows no cross-reactivity with recombinant cytokines from other species: rat TNF, mouse TNF, mouse TNFRI, mouse TNFRII.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
