BD, 553392, Purified Rat Anti-Mouse IgG2b R12-3

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
For the sandwich mouse, Biotin Rat Anti-Mouse IgG2b (Cat. No. 553393) is optimal for detection with Purified Rat Anti-Mouse IgG2b (Cat. No. 553396) for capture. Purified Rat Anti-Mouse IgG2b may be used as a primary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC Rat Anti-Mouse IgG2b (Cat. No. 553395).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.