BD, 553393, Biotin Rat Anti-Mouse IgG2b R12-3

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
ELISA: For the mouse IgG2b sandwich ELISA, Biotin Rat Anti-Mouse IgG2b is optimal for detection (~2 µg/ml) using Purified Rat Anti-Mouse IgG2b, (Cat. No. 553396) for capture, with Purified Mouse IgG2b, κ Isotype Control, (Cat. No. 557351) as the standard. Immunofluorescent staining: Biotin Rat Anti-Mouse IgG2b may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC Rat Anti-Mouse IgG2b, (Cat. No. 553395). Immunohistochemistry: For IHC, we recommend the use of Biotin Rat Anti-Mouse IgG2b, (Cat. No. 550333) in our special formulation for immunohistochemistry.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.