BD, 553432, Purified Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain R26-46

The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.
For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553433) cocktailed with Biotin Rat Anti-Mouse Ig, κ Light Chain (Cat. No. 559750) is optimal for detection with any of the following anti-mouse Ig isotype-specific mAbs: Purified Rat Anti-Mouse IgG1 (Cat. No. 553440); Purified Rat Anti-Mouse IgG2a (Cat. No. 553387); Purified Rat Anti-Mouse IgG2b (Cat. No. 553392); R40-82 [available by custom order]); Purified Rat Anti-Mouse IgM (Cat. No. 553435); Purified Rat Anti-Mouse IgA (Cat. No. 556969); Purified Rat Anti-Mouse IgE (Cat. No. 553413) for capture, respectively.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.