BD, 553445, Purified Rat Anti-Mouse IgG1 A85-3

The A85-3 antibody reacts specifically with mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes.
ELISA: The Purified Rat Anti-Mouse IgG1 antibody (clone A85-3) may be used as a capture reagent with Biotin Rat Anti-Mouse IgG1 (clone A85-1) (Cat. No. 553441) and Avidin-HRP (Cat. No. 554058) or Streptavidin-HRP (Cat. No. 554066) as detection reagents. Alternatively, investigators may also wish to consider using AKP Rat Anti-Mouse IgG1 (Cat. No. 557272) or HRP Rat Anti-Mouse IgG1 (Cat. No. 559626) for detection. Purified Mouse IgG1 (Cat. No. 557273) should be used as the protein standard.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.