BD, 553515, Biotin Mouse Anti-Mouse IgM[a] DS-1

The DS-1 antibody reacts specifically with mouse IgM of Igh-C[a] and related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2). It does not react with IgM of Igh-C[b], Igh-C[e], or related hapltypes (e.g., C57BL, SJL, A, AKR, NZB). DS-1 antibody does not react with free µ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.
Biotin DS-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.