BD, 554005, Purified NA/LE Mouse Anti-Armenian and Syrian Hamster IgG1 G94-56

Based on ELISA, the G94-56 antibody reacts specifically with Armenian and Syrian hamster IgG1 monoclonal antibodies, but has low reactivity with some other hamster IgG groups. The G94-56 mAb does not react with hamster IgM.
ELISA: For sandwich ELISA (hamster IgG (group 1)), biotin-conjugated mAb G94-56 (Cat. No. 554007) is optimal for detection with purified anti-hamster IgG mAb HIG-632 (Cat. No. 550637) for capture.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.