BD, 554385, Purified NA/LE Rat Anti-Mouse IL-4 BVD4-1D11

The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4.
ELISA: The purified format of BVD4-1D11 antibody (Cat. No. 554387) can be useful as a capture antibody in a sandwich ELISA for measuring mouse IL-4. Purified BVD4-1D11 antibody can be paired with the biotinylated BVD6-24G2 antibody (Cat. No. 554390) as the detecting antibody, with recombinant mouse IL-4 (Cat. No. 550067) as the standard. For testing mouse IL-4 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse IL-4 Set (Cat. No. 555232).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.