Description
The XMG1.2 monoclonal antibody specifically binds to mouse interferon-γ (IFN-γ) protein. IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-γ production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
Immunofluorescent Staining and Flow Cytometric Analysis: The purified XMG1.2 antibody can be used as a blocking control to demonstrate specificity of IFN-γ staining by directly conjugated-XMG1.2 (PE: Cat. No. 554412; FITC: Cat. No. 554411; APC: Cat. No. 554413; PE-Cy7: Cat. No. 557649). To perform this control, the fixed/permeabilized cells (~1 million) can be incubated with 1-10 µg of unlabeled XMG1.2 antibody (Cat. No. 554409) for 20 minutes at 4°C, prior to staining with conjugated-XGM1.2 antibody. Western blot: The purified XMG1.2 antibody (Cat. No. 554409) has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen. ELISA Detection: The biotinylated XMG1.2 antibody (Cat. No. 554410) is useful as a detection antibody for a sandwich ELISA for measuring mouse IFN-γ protein levels and can be used in combination with purified R4-6A2 (Cat. No. 551216) as the capture antibody and recombinant mouse IFN-γ as the standard. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring Mouse IFN-γ in serum or plasma our Mouse IFN-γ BD OptEIA™ Set (Cat. No. 551866) or BD OptEIA Kit (Cat. No. 558258) are specially formulated and recommended. Neutralization/Blocking: The NA/LE™ format of the XMG1.2 antibody (Cat. No. 554408) is useful for neutralization of mouse IFN-γ bioactivity.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
