Description
The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the JES6-1A12 hybridoma was recombinant mouse IL-2. This is a neutralizing antibody.
1. ELISA Capture: The purified JES6-1A12 antibody (Cat. No. 554424) is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-2 protein levels. Purified JES6-1A12 antibody can be paired with the biotinylated JES65H4 antibody (Cat. No. 554426) as the detecting antibody, with recombinant mouse IL-2 (Cat. No. 550069) as the standard. Purified JES6-1A12 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of mouse IL-2 ranging from ~500 to 4 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. 2. IP/WB: The JES6-1A12 antibody (Cat. No. 554424) has been reported to be useful for immunoprecipitation studies and for Western blotting. Please note that this application is not routinely tested at BD Biosciences. 3. IF/Flow: For immunofluorescent staining and flow cytometric analysis, the JES6-5H4 antibody has been found useful to identify and enumerate IL-2 producing cells within mixed cell populations. Directly conjugated JES6-5H4 antibodies are especially suitable for these studies. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. 4. Neutralization: The NA/LE™ S4B6 antibody (Cat. No. 554375) is useful for neutralization of mouse IL-2 bioactivity. A suitable NA/LE rat IgG2a isotype-matched control is the R35-95 antibody (Cat. No. 554687).
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
