BD, 554430, Purified NA/LE Rat Anti-Mouse IFN-γ R4-6A2

The R4-6A2 antibody reacts with mouse interferon-γ (IFN-γ). The immunogen used to generate the R4-6A2 hybridoma was partially purified mouse IFN-γ protein. This is a neutralizing antibody.
ELISA: Purified Rat Anti-Mouse IFN-γ (Clone R4-6A2, Cat. No. 551216) has been reported to be useful as a capture antibody for sandwich ELISA measuring mouse IFN-γ protein levels. Purified R4-6A2 antibody can be paired with Biotin Rat Anti-Mouse IFN-γ (XMG1.2, Cat. No. 554410) as the detection antibody and using recombinant mouse IFN-γ (Cat. No. 554587) as the standard. For measuring mouse IFN-γ in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IFN-γ ELISA Set (Cat. No. 551866) or BD OptEIA™ Mouse IFN-γ ELISA Kit II (Cat No. 558258).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.