BD, 554475, Purified NA/LE Rat Anti-Mouse IL-12 (p40/p70) C17.8

The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
ELISA (IL-12 p70 Detection): Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p70 antibody (Clone 9A5, Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard. For measuring mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p70 ELISA Set (Cat. No. 555256). ELISA (IL-12 p40 Detection): Biotin Rat Anti-Mouse IL-12 (p40/p70) antibody (Clone C17.8, Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the Purified Rat Anti-Mouse IL-12 p40/p70 antibody (Clone C15.6, Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard. For measuring mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-12 p40 ELISA Set (Cat. No. 555165).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.