BD, 554508, Purified NA/LE Mouse Anti-Human TNF MAb1

The MAb1 antibody reacts with human tumor necrosis factor (TNF, aka TNF-α) protein. TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer.
ELISA: Purified Mouse Anti-Human TNF antibody (Clone MAb1, Cat. No. 551220) is useful as a capture antibody for a sandwich ELISA for measuring human TNF. The purified MAb1 antibody can be paired with Biotin Mouse Anti-Human TNF mAb ( Clone MAb11, Cat. No. 554511) as the detection antibody, with recombinant human TNF protein (Cat. No. 554618) as the standard. For complex biological samples, such as serum or plasma, investigators are encouraged to use the BD OptEIA™ Human TNF ELISA Set (Cat. No. 555212) or BD OptEIA™ Human TNF ELISA Kit II (Cat. No. 550610).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.