Description
The MQ1-17H12 monoclonal antibody specifically binds to the multifunctional cytokine, human Interleukin-2 (IL-2). IL-2 is produced by activated T cells and has multiple functions that can affect the growth, proliferation, differentiation and survival of many different target cell types including T cells, B cells, NK cells, monocytes and macrophages. The immunogen used to generate the MQ1-17H12 hybridoma was purified recombinant human IL-2 protein. The MQ1-17H12 antibody reportedly neutralizes the biological activity of human IL-2.
Blocking Control for Intracellular Staining: The purified MQ1-17H12 antibody (Cat. No. 554563) can be used as a blocking control to demonstrate specificity of IL-2 staining of directly conjugated MQ1-17H12 antibody. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 -10 µg of unlabeled MQ1-17H12 antibody (Cat. No. 554563) for 20 minutes at 4°C, prior to staining with conjugated antibody (e.g., 0.1 -0.5 µg mAb/1X10^6 cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocol section on our web site, http://www.bdbiosciences.com/resources/index.jsp Neutralization/Blocking: THE NA/LE™ format of the MQ1-17H12 antibody (Cat. 554562) is useful for neutralization of human IL-2 bioactivity. A suitable NA/LE™ isotype control to match the NA/LE™ MQ1-17H12 antibody is the R35-95 antibody (Cat. No. 554687). ELISA Capture: The purified MQ1-17H12 antibody is useful as a capture antibody for a sandwich ELISA for specifically measuring human IL-2 protein levels. The biotinylated B33-2 antibody (Cat. No. 555040) can be paired with the purified MQ1-17H12 antibody (Cat. No. 554563) as the capture antibody, with recombinant human IL-2 (Cat. No. 554603) as the standard. For specific methodology, please visit the protocols section on our website, http://www.bdbiosciences.com/resources/index.jsp
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Cy is a trademark of GE Healthcare.
6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
