Description
Upon initial thawing, recombinant mouse MCP-1 (MN 554590) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity. ELISA Standard: Recombinant mouse MCP-1 (MN 554590) can be useful as a quantitative standard for measuring mouse MCP-1 protein levels using sandwich ELISA with the purified 2H5 antibody (Cat. No. 551217) as a capture antibody and the biotinylated 4E2/MCP antibody (Cat. No. 554444) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant mouse MCP-1 from 4000-30 pg/mL to be included for each ELISA plate. For measuring mouse MCP-1 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse MCP-1 Set (Cat. No. 555260). Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range encompassing an ED50 = 50 - 800 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay.
Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
