Description
ELISA Standard: This recombinant human IL-12 p40 protein is useful as a quantitative standard for an IL-12 p40 sandwich ELISA. For this assay, the purified C8.3 capture antibody (Cat. No. 551227) can be paired with the biotinylated C8.6 detection antibody (Cat. No. 554660). For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. Note 1: The ELISA antibody pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human IL-12p40 in serum or plasma our Human IL-12p40 OptEIA™ Set (Cat. No. 555171 ) or OptEIA Kit II (Cat. No. 551116) is specially formulated and recommended. Note 2: Carrier proteins** should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
