Description
The BVD8-3G11 antibody reacts with human interleukin-3 (IL-3). The immunogen used to generate the BVD8-3G11 hybridoma was yeast-expressed recombinant human IL-3. This is a neutralizing antibody.
ELISA Capture: The purified BVD8-3G11 antibody (Cat. No. 554672) is useful as a capture antibody in a sandwich ELISA for measuring human IL-3 protein levels. Purified BVD8-3G11 antibody can be paired with the biotinylated BVD3-1F9 antibody (Cat. #554674) as the detecting antibody, with recombinant human IL-3 (Cat. No. 554604) as the standard. This purified capture antibody should be pretitrated in the range of 1 - 4 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of human IL-3 ranging from ~2,000 to 15 pg/ml should be used. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum samples. For testing human IL-3 in complex biological fluids such as serum or plasma, our BD OptEIA™ Human IL-3 ELISA Set (Cat. No. 555228) is recommended. WB: The BVD8-3G11 antibody has been found useful for western blotting. A concentration of 1-5 µg/ml (in conjunction with the AKP-conjugated goat anti-rat Igs has been found to enable visualization of ≤ 100 ng/lane of recombinant IL-3 (e.g., Cat. No. 554604), under reducing conditions. Neutralization: The BVD8-3G11 antibody (Cat. No. 554671) is useful for neutralization of human IL-3 bioactivity.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
