Description
The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was recombinant human IL-8.
ELISA Capture: For a sandwich ELISA to measure human IL-8 protein levels, the Purified Mouse Anti-Human IL-8 (Cat. No. 554716) can be paired with the Biotin Mouse Anti-Human IL-8 (Cat. No. 554718), with Recombinant Human IL-8 (Cat. No. 554609) as the standard. Purified Mouse Anti-Human IL-8 should be titrated from 1 - 4 µg/ml to determine the optimal concentration for ELISA capture. To obtain standard curves, doubling dilutions of human IL-8 protein ranging from ~1,000 to 1 pg/ml are recommended for inclusion in each ELISA plate. It is recommended that the investigator should prepare dilutions of the sample to ensure that the level of IL-8 falls within this range. For specific methodology, please visit the protocols under "ELISA and ELISPOT" at our website, http://www.bdbiosciences.com/us/s/resources. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring mouse IL-8 in serum or plasma our mouse IL-8 BD OptEIA™ Set (Cat. No. 555244) or BD OptEIA Kit (Cat. No. 550999) are specially formulated and recommended. This ELISA pair shows no cross-reactivity with any other cytokines (e.g., human IL-1α, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFN-γ, TNF, LT-α, MCP-1, MCP-2, MIP-1α, MIP-1ß, SCF, lymphotactin, VEGF, G-CSF, NT-3, PDGF, CD23, IP-10, GRO, NAP-2, PF4). Western Blot: The clone G265-5 has been reported to be useful for Western Blotting. Please note that this application is not routinely tested at BD Biosciences.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
