The monoclonal antibody B8-6 reacts with human monokine induced by gamma interferon (MIG). MIG is inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ, but not by IFN-α or bacterial lipopolysaccharides. The immunogen used to generate the monoclonal antibody B8-6 was insect cell-expressed, recombinant human MIG protein.
ELISA Detection: The biotinylated B8-6 antibody (Cat. No. 555037) is useful as a detection antibody in a sandwich ELISA for measuring human MIG protein levels. The biotinylated B8-6 antibody can be paired with the purified monoclonal antibody B8-11 (Cat. No. 555038) with recombinant human MIG (Cat. No. 554636) as the standard. The biotinylated B8-6 antibody should be titrated to determine optimal concentration for ELISA detection (2.0 - 6.0 µg/ml). To obtain linear standard curves, doubling dilutions of human MIG ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. This ELISA pair shows no cross-reactivity with any of the cytokines tested ( human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, IP-10, lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TNF, LT-β). This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum samples. The OptEIA™ Human MIG ELISA Set (Cat. No. 550998) is specially-formulated for serum cytokine measurement.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
