BD, 555280, Purified NA/LE Rat Anti-Mouse CD18 GAME-46

The GAME-46 monoclonal antibody specifically binds to CD18. CD18 is the common β2 integrin chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αXβ2 integrin). Expression of CD18 is limited to leucocytes, where it is widely distributed in consort with each of the three integrin α chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The β2 integrins are important mediators of leukocyte-endothelium interactions. It has been reported that GAME-46 antibody blocks in vitro adhesion of LFA-1-expressing cells to the ligands ICAM-1, ICAM-2 and ICAM-3 and of Mac-1-expressing cells to ICAM-1, C3bi, and fibrinogen.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.