BD, 555612, Purified Mouse Anti-Human CD74 LN2 (also known as LN-2)

The LN2 monoclonal antibody specifically recognizes CD74, the cell surface MHC class II-associated invariant chain molecule (41, 35, 33 kDa). CD74 is moderately expressed on B cells, and weakly on monocytes and macrophages. Other reactive cells include Langerhans cells, dendritic cells, a subset of activated T cells and thymic epithelium. The LN2 monoclonal antibody labels a nuclear membrane protein of follicular center and mantle zone B cells and a cytoplasmic protein of cells which bind HLA class II antibodies. CD74 is postulated to play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections without citrate pretreatment.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.