BD, 555663, Purified Mouse Anti-Human CD86 IT2.2

The IT2.2 monoclonal antibody specifically binds to CD86. CD86 is a ~ 75 kDa type I transmembrane glycoprotein which is also known as B7-2 or B70. CD86 is primarily expressed on monocytes, dendritic cells, Langerhans cells, and activated B cells including B lymphoid cells in germinal centers and Epstein-Barr virus transformed B-cell lines. CD86 is a ligand for CD28 and CD152 (CTLA-4) and may play an important role in costimulation of T cells in primary immune responses. Competitive binding assays demonstrate that, while both the IT2.2 and FUN-1 monoclonal antibodies recognize the same CD86 molecule, they react with different epitopes. IT2.2 blocks the costimulation activity of CD86 in functional studies and blocks binding of human CTLA-4-Ig fusion protein to CD86 gene-transfected cells.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.