BD, 555685, Purified Mouse Anti-Human CD89 A59

The A59 monoclonal antibody specifically recognizes CD89. CD89 is the Fc receptor for IgA (FcαR), a 55-75 kDa glycoprotein expressed exclusively on cells of granulocytic and monocyte/macrophage lineages in peripheral blood, but not on lymphocytes. It is also expressed on promyelocytes in bone marrow and in the cytoplasm of fixed monocytes. This mAb does not block IgA binding and is more efficient than native IgA ligands in the isolation of FcαR molecules. FcαR plays a role in triggering phagocytosis and respiratory burst.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.