BD, 556039, Purified Mouse Anti-Human CD109 TEA 2/16

The TEA 2/16 monoclonal antibody specifically recognizes CD109 which is also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 (CPAMD7), Gov platelet alloantigens, or 150 kDa TGF-beta-1-binding protein. CD109 is a 150-170 kDa glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on activated T cells, myeloid progenitor (CD34+) and mature myeloid lineage cells (monocytes, granulocytes, platelets), but not on CD34+ lymphoid progenitor cells. CD109 is also expressed on vein and artery endothelial cells. The expression of CD109 is upregulated on PHA-stimulated T cells. CD109 is reportedly expressed on long-term adult bone marrow cultured cells, where it is co-expressed with CD34 and CD90. Its biological role in hematopoiesis has not been fully elucidated. It may play a role in the negative regulation of transforming growth factor beta receptor signaling.

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.