BD, 556960, Purified Rat Anti-Mouse IgA C10-1

The C10-1 antibody reacts specifically with mouse IgA of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with C10-1 mAb.
For the sandwich mouse, Biotin Rat Anti-Mouse IgA (Cat. No. 556978) is optimal for detection with Purified Rat Anti-Mouse IgA (Cat. No. 556969) for capture. Purified Rat Anti-Mouse IgA (Cat. No. 556960) may be used as a primary reagent in immunofluorescent staining and immunohistochemical staining of acetone-fixed frozen sections. For flow cytometric detection of intracytoplasmic IgA, we recommend FITC Rat Anti-Mouse IgA (Cat. No. 559354).
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.