Description
The I87-419 antibody reacts with the mouse C3a protein. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent. The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.
Biotin Rat Anti-Mouse C3a antibody (Cat. No. 558251) is useful as a detector antibody for a sandwich ELISA that measures mouse C3a protein levels. Purified Rat Anti-Mouse C3a antibody (Cat. No. 558250) can be used as the capture antibody and paired with Biotin Rat Anti-Mouse C3a antibody as the detection antibody, using purified mouse C3a protein as standard (Cat. No. 558618). Purified Rat Anti-Mouse C3a antibody should be titrated between 0.25-2 µg/ml to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse C3a protein ranging from 781 pg/ml to 50 ng/ml are recommended for inclusion in each ELISA plate. Addition of FUT-175 (Futhan) to plasma samples at the time of sample collection provides additional protection from ex-vivo activation, and therefore ensures more accurate measurements that reflect the circulating levels of complement activation products. For specific methodology measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors, see the protocols under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources. For this ELISA antibody pair, no cross-reactivity has been observed with the following: recombinant mouse C5a, native human C3a, C4a and C5a, and rat C5a. This ELISA antibody pair shows a limited cross-reactivity with rat C3a.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
