Description
The BD™ CBA Human CD178 (Fas Ligand) Flex Set must be used in conjunction with a BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD™ CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD™ CBA Human CD178 (Fas Ligand) Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1. When multiplexing the BD™ CBA Human CD178 (Fas Ligand) Flex Set assay with the BD™ CBA Human LT-α Flex Set assay (Cat. No. 560083), significantly higher background will be seen in the human Fas Ligand assay. This increased background, while reducing the sensitivity of the human Fas Ligand assay, will not effect the quantitation of human Fas Ligand or LT-α in any other way. The BD™ CBA Human CD178 (Fas Ligand) Flex Set cannot be used in the same assay well with the following BD™ CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD™ CBA Human OSM Flex Set D5 560084 The BD™ CBA Human CD178 (Fas Ligand) Flex Set should not be used in the same assay well with any non-BD™ CBA Human Soluble Protein Flex Set reagents (such as BD™ CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD™ CBA Human Soluble Protein Flex Sets, please refer to the BD™ CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset. Performance Limit of Detection: The theoretical limit of detection is 2.6 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.
1. ProClin is a trademark of Rohm and Haas Company.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
