Description
The BD CBA Human IL-3 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human IL-3 Flex Set will be 2,500 pg/mL. An example standard curve is shown in Figure 1. When multiplexing the BD CBA Human IL-3 Flex Set assay with the BD CBA Human IL-7 Flex Set assay (Cat. No. 558334), BD CBA Human IL-8 Flex Set assay (Cat. No. 558277), BD CBA Human IL-9 Flex Set assay (Cat. No. 558333) and BD CBA Human IL-10 Flex Set assay (Cat. No. 558274), significantly higher background will be seen in the human IL-3 assay. This increased background, while reducing the sensitivity, will not effect the quantitation of human IL-3 in any other way. There is no effect on the human IL-7, IL-8, IL-9 or IL-10 assays. The BD CBA Human IL-3 Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human IL-12/IL-23p40 Flex Set E5 560154 BD CBA Human LT-α Flex Set D5 560083 BD CBA Human OSM Flex Set D5 560084 The BD CBA Human IL-3 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup. Performance Limit of Detection: The theoretical limit of detection is 0.3 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.
This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
1. ProClin is a trademark of Rohm and Haas Company.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
