Description
The I76-539 antibody reacts with interleukin-21 (IL-21), an ~ 15 kDa member of the type-I-cytokine family of proteins. IL-21 is expressed primarily by CD4+ T cells and has a broad range of immunoregulatory functions on B cells, CD8+ T cells, NK cells and dendritic cells. The effects of IL-21 include promotion of antibody production, NK cell differentiation, proliferation and differentiation of cells of monocyte and granulocyte lineages, costimulation of T cells and augmentation of antitumor activity of CD8+ T cells. It has been speculated that IL-21, through its varies positive and negative regulatory effects on cell-mediated and humoral immunity, may play a role in autoimmuninty, allergy, asthma and tumor regression.
The biotinylated I76-539 mAb is useful as a detection antibody for a sandwich ELISA that measures human IL-21 protein when paired with purified mouse anti-human IL-21 (Cat. No. 558503) as capture antibody and recombinant human IL-21 protein as standard. The biotinylated I76-539 antibody should be titrated between 1 - 4 µg/ml to determine its optimal concentration. To obtain linear standard curves, doubling dilutions of recombinant human IL-21 protein ranging from 0.39 - 50 ng/ml are recommended. For specific methodology, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis Application Handbook 1st Edition. BD Biosciences. For this ELISA antibody pair, no cross-reactivity has been observed with the following: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12/23p40, IL-12p70, IL-13, IL-15, IFN-γ, G-CSF, M-CSF, TNF and TGF-β.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
