Description
The BD CBA Human IgE Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. The top standard point for the BD CBA Human IgE Flex Set is 500 ng/ml and should be prepared in the following way: For single-plex Human IgE Flex Set experiments, make the top standard dilution by mixing 20µl of the Human IgE Standard with 980µl of Assay Diluent from the Human Soluble Protein Master Buffer Kit in a tube labeled Top Standard. Perform additional standard dilutions using the top standard dilution by following the instructions in the Human Soluble Protein Master Buffer Kit manual. For multi-plex experiments including the Human IgE Flex Set, first reconstitute all the other analytes (lyophilized standard proteins) as directed in the Human Soluble Protein Master Buffer Kit manual, but use 20µl less Assay Diluent per ml used for reconstitution (e.g., if reconstituting lyophilized pellets in 1 ml of Assay Diluent, use 980µl instead). Transfer 980µl of the reconstituted proteins to a tube labeled Top Standard and add 20µl of the Human IgE Standard. Perform additional standard dilutions using the top standard dilution by following the instructions in the Human Soluble Protein Master Buffer Kit manual. An example standard curve is shown in Figure 1. The BD CBA Human IgE Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein, Human Immunoglobulin or Cell Signaling Flex Sets). The BD CBA Human IgE Flex Set can not be used in the same assay well with any other Human immunoglobulin Flex Set assay or with the Human Ig Master Buffer Kit. For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset. Performance Limit of Detection: The theoretical limit of detection is 0.07 ng/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
2. This product contains human blood, serum, cells, or materials derived from them, which are potentially hazardous materials. Use universal precautions when handling. Handle as if product were capable of transmitting disease. Material used in this product has been tested using FDA approved methods and found negative for Human Immunodeficiency Virus (HIV-1/HIV-2), Hepatitis B Surface Antigen (HBSAG) and antibody to Hepatitis C Virus (HCV). However, no known test method can offer complete assurance that specimens of human origin will not transmit infectious disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen recommendations.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
