BD, 559047, Purified Mouse Anti-Human CD54 LB-2

The LB-2 monoclonal antibody specifically binds to CD54 which is also known as Intercellular adhesion molecule-1 (ICAM-1). CD54 is an 85-110 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin supergene family. A soluble form of CD54 can also be found in biological fluids. CD54 is expressed on endothelial cells and both resting (weak) and activated (moderate) T and B lymphocytes and monocytes. CD54 is expressed on human myeloma cells, Burkitt's lymphoma cells, erythroleukemia cells, and B-lymphoblastoid cells. CD54 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) and can act as a receptor for Rhinoviruses. The CD54 adhesion molecule plays roles in inflammatory and immune responses and neoplasia.
Functional Studies: ICAM-1 is a ligand for both the leukocyte function-associated antigen-1 (LFA-1) and Mac-1, and thereby mediates the adhesion of leukocytes to endothelial cells. Leukocyte binding to endothelial cells is a critical process in inflammatory responses such as rheumatoid arthritis, adult respiratory distress syndrome, chronic dematoses, and reperfusion myocardial injury. Anti-ICAM-1 monoclonal antibody (clone LB-2) inhibits phorbol ester-enhanced aggregation of EBV-B or lectin-activated normal blood mononuclear cells. Anti-ICAM-1 (clone LB-2) is specific for a functional epitope on ICAM-1 and inhibits LFA-1-ICAM-1 interactions. Immunoprecipitation: Under reducing conditions, anti-ICAM-1 monoclonal antibody (clone LB-2) immunoprecipitates a glycoprotein from EBV-immortalized B lymphocytes (EBV-B) with a molecular mass of 84 kDa by gel electrophoresis, while under nonreducing conditions a molecular mass of 77 kDa was observed. Immunohistology: Anti-ICAM-1 monoclonal antibody (clone LB-2) can be used for immunohistochemistry staining.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.