BD, 560104, Assay Diluent

Name: Assay Diluent
Brand: BD
SKU: 560104

30 ML

Catalog Number: 560104

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Category

Chemiluminescence Immunoassay Reagents

TagsReagents immunoassay-reagents

CollectionsBD Single-Cell Multiomics Reagents for the BD Rhapsody™ Platform BD Immunoassay Reagents: CBA, ELISA & ELISPOT for Cytokine Panels BD Cell Preparation & Separation Reagents (PBMC, Magnetic, RBC Lysis)

Description

For detailed instructions on the use of the BD CBA Assay Diluent, refer to the instruction manual of the appropriate BD CBA Master Buffer Kit (see Suggested Companion Products). The BD CBA Assay Diluent cannot be used with the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006) nor with any of the BD CBA Cell Signaling Flex Sets and BD CBA Kit products.
Store undiluted at 4°C.
1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Specifications

General

Source	Recombinant human PD-L1, amino acid (Phe19-Arg238) (Accession: #Q9NZQ7.1), with a C-terminal 6x His, was expressed in 293E cells.
Molecular Mass	The 226 amino acid recombinant protein has a predicted molecular mass of approximately 26.01 kD. The DTT-reduced and non-reduced protein migrates at approximately 32-40 kD by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity	> 98%, as determined by Coomassie stained SDS-PAGE
Formulation	0.22 µm filtered protein solution is in PBS
Endotoxin Level	Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration	25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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