BD, 560106, APC Rat anti-Mouse CD172a P84
0.1 mg
Catalog Number: 560106
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| Source | Recombinant human PD-L1, amino acid (Phe19-Arg238) (Accession: #Q9NZQ7.1), with a C-terminal 6x His, was expressed in 293E cells. |
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| Molecular Mass | The 226 amino acid recombinant protein has a predicted molecular mass of approximately 26.01 kD. The DTT-reduced and non-reduced protein migrates at approximately 32-40 kD by SDS-PAGE. The predicted N-terminal amino acid is Phe. |
| Purity | > 98%, as determined by Coomassie stained SDS-PAGE |
| Formulation | 0.22 µm filtered protein solution is in PBS |
| Endotoxin Level | Less than 0.1 EU per µg cytokine as determined by the LAL method. |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling | Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
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| Activity | Biotinylated recombinant human PD-1 (Cat. No. 789402) binds to immobilized recombinant human PD-L1 in a dose-dependent manner. Recombinant human PD-L1 was immobilized using purified anti-His Tag antibody (Cat. No. 652501). HRP-Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.1 – 0.6 µg/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell |
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| Function | Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance |
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| Interaction | Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes |
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| Ligand/Receptor | PD-1 (CD279) |
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| Bioactivity | Tested by ability of PD-L1 to bind to its ligand PD-1 |
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| Cell Type | Antigen-presenting cells, Dendritic cells, Monocytes |
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| Biology Area | Cell Proliferation and Viability, Immuno-Oncology, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Dong H, et al. 1999. Nat Med. 5:1365-9. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34. Latchman Y, et al. 2001. Nat Immunol. 2:261-8. Latchman YE, et al. 2004. Proc Natl Acad Sci USA. 101:10691-6. Guleria I, et al. 2005. J Exp Med. 202:231-7. Lin DY, et al. 2008. Proc Natl Acad Sci USA. 105:3011-6. Dai S, et al. 2014. Cell Immunol. 290:72-9. Melero I, et al. 2015. Nat Rev Cancer. 15:457-72. |
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| Gene ID | 29126 |
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| UniProt | View information about PD-L1 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1) |
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