BD, 560268, Purified NA/LE Rat Anti-Mouse IL-17A TC11-18H10.1

The TC11-18H10 monoclonal antibody specifically binds to recombinant and natural mouse IL-17A proteins. IL-17A, also known as CTLA-8, is a T cell-derived cytokine that promotes inflammatory responses. Mouse IL-17A is a proinflammatory cytokine that can induce the release of IL-6 by mouse stromal cells. It has been shown to support the growth of hemopoietic progenitors in vitro; it can also stimulate granulopoiesis in vivo. The TC11-18H10 antibody has been reported to neutralize IL-17A activity. Recent studies have shown that IL-17A is produced by a unique subset of Th17 cells that develop along a pathway distinct from the Th1- and Th2- cell differentiation pathways. The mouse IL-17A cDNA was isolated from a cDNA library generated from TCRαβ+CD4-CD8- thymocytes.
ELISA: Purified TC11-18H10 antibody (Cat. No. 560268) can be useful as a capture antibody for sandwich ELISA measuring mouse IL-17A protein levels. Purified TC11-18H10 antibody can be paired with biotinylated TC11-8H4 (Cat. No. 555067) as the detector antibody, with recombinant mouse IL-17A protein as the standard. Investigators are encouraged to titrate purified TC11-18H10 from 2.0 µg/mL - 0.5 µg/mL for optimal concentration determination for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant mouse IL-17A are suggested from 2,000 - 15 pg/mL.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.